Coding

Part:BBa_K2558003:Design

Designed by: Tianze Huang   Group: iGEM18_Tsinghua   (2018-10-02)


dCas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3378
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence is modified at A1341T in order to be RFC10 compatible.


Source

From collaboration with Chen Lab.

References